Proteomics Core Facility
The UMKC Proteomics Core Facility provides Liquid Chromatography and Mass Spectrometry services for proteomic analysis studies of UMKC researchers and external users.
Lab Capabilities
The Core Facility is equipped with a Vanquish Neo Nano-UHPLC and an Orbitrap Eclipse Tribrid Mass Spectrometer fitted with a Faims Pro Duo for extended differential ion mobility. This system has extremely high resolution and is capable of isolating, identifying and quantifying proteins from both simple and complex samples.
Analysis of small molecule products for quality control, validation/proof of synthesis or organic synthesis troubleshooting is also available.
The Core Facility houses a full complement of ancillary lab equipment for sample processing, with an approved IBC protocol for BSL2 level work with a certified biosafety cabinet for handling clinical samples safely. Data security is ensured by both on-site and cloud-based data backup systems.
Lab Services
Please contact us for current rates.
- Full-Service Routine Sample Prep and LC/MS Protein ID
- Full-Service Routine Sample Prep and LC/MS Protein ID with Label-free Quantification
- Full-Service Routine Sample Prep and LC/MS Protein ID with multi-plex TMT Label Quantification
- LC/MS Protein ID, Raw Data Only
- Abundant Protein Depletion (Per Sample)
- Albumin Depletion (Per Sample)
- Post-Translational Modification (PTM) Scan Analysis
- Consulting – Project Development and Data analysis
- Advanced Sample Prep
- Standard Data Analysis
- Unassisted MS time
Equipment
Orbitrap Eclipse Tribrid Mass Spectrometer with Faims Pro Duo and EasySpray NG ion source (ThermoFisher Scientific)
Vanquish Neo UHPLC capable of nano, capillary, and micro flow LC
Data Analysis
Proteomics Data Search and analysis software available:
- Proteome Discoverer 2.5
- Mascot Server
- MaxQuant
- Perseus
- MSFragger
Getting Started
Please contact us if you would like to discuss a potential project.
Sean Riordan, Ph.D.Core Facility Director
816-235-6774